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Promega 5×amv rt reaction buffer
5×Amv Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+amv-rt+buffer/5%C3%97+amv+rt+buffer/pmc00034109-126-6-28
Average 90 stars, based on 1 article reviews
5×amv rt reaction buffer - by Bioz Stars, 2026-10
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Related Articles

Primer Extension Assay:

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA
Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Article Title: Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containingMycobacterium tuberculosis stress response σ factor, σF
Article Snippet: The previously established two-plasmid system in Escherichia coli for the identification of Mycobacterium tuberculosis promoters that are recognized by RNA polymerase containing the stress response factor F was optimized.. Expression of the M. tuberculosis F encoded by sigF gene was under the control of the isopropyl -D-thiogalactopyranoside (IPTG)-dependent Ptrc promoter.. A low level of IPTG induced a nontoxic but sufficient level of F to interact with the core enzyme of RNA polymerase.

Article Title: Mapping of transcription start sites of human retina expressed genes
Article Snippet: First strand cDNA synthesis was performed using Avian Myeloblastoma Virus (AMV) reverse transcriptase and 5× AMV-RT buffer (Promega) according to the manufacturer's instructions in a final volume of 60 μl.

Labeling:

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA
Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Article Title: Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containingMycobacterium tuberculosis stress response σ factor, σF
Article Snippet: The previously established two-plasmid system in Escherichia coli for the identification of Mycobacterium tuberculosis promoters that are recognized by RNA polymerase containing the stress response factor F was optimized.. Expression of the M. tuberculosis F encoded by sigF gene was under the control of the isopropyl -D-thiogalactopyranoside (IPTG)-dependent Ptrc promoter.. A low level of IPTG induced a nontoxic but sufficient level of F to interact with the core enzyme of RNA polymerase.

Article Title: Mapping of transcription start sites of human retina expressed genes
Article Snippet: First strand cDNA synthesis was performed using Avian Myeloblastoma Virus (AMV) reverse transcriptase and 5× AMV-RT buffer (Promega) according to the manufacturer's instructions in a final volume of 60 μl.

Inhibition:

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA
Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Article Title: Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containingMycobacterium tuberculosis stress response σ factor, σF
Article Snippet: The previously established two-plasmid system in Escherichia coli for the identification of Mycobacterium tuberculosis promoters that are recognized by RNA polymerase containing the stress response factor F was optimized.. Expression of the M. tuberculosis F encoded by sigF gene was under the control of the isopropyl -D-thiogalactopyranoside (IPTG)-dependent Ptrc promoter.. A low level of IPTG induced a nontoxic but sufficient level of F to interact with the core enzyme of RNA polymerase.

Article Title: Mapping of transcription start sites of human retina expressed genes
Article Snippet: First strand cDNA synthesis was performed using Avian Myeloblastoma Virus (AMV) reverse transcriptase and 5× AMV-RT buffer (Promega) according to the manufacturer's instructions in a final volume of 60 μl.

Standard Deviation:

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA
Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Article Title: Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containingMycobacterium tuberculosis stress response σ factor, σF
Article Snippet: The previously established two-plasmid system in Escherichia coli for the identification of Mycobacterium tuberculosis promoters that are recognized by RNA polymerase containing the stress response factor F was optimized.. Expression of the M. tuberculosis F encoded by sigF gene was under the control of the isopropyl -D-thiogalactopyranoside (IPTG)-dependent Ptrc promoter.. A low level of IPTG induced a nontoxic but sufficient level of F to interact with the core enzyme of RNA polymerase.

Article Title: Mapping of transcription start sites of human retina expressed genes
Article Snippet: First strand cDNA synthesis was performed using Avian Myeloblastoma Virus (AMV) reverse transcriptase and 5× AMV-RT buffer (Promega) according to the manufacturer's instructions in a final volume of 60 μl.

cDNA Synthesis:

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA
Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Article Title: Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containingMycobacterium tuberculosis stress response σ factor, σF
Article Snippet: The previously established two-plasmid system in Escherichia coli for the identification of Mycobacterium tuberculosis promoters that are recognized by RNA polymerase containing the stress response factor F was optimized.. Expression of the M. tuberculosis F encoded by sigF gene was under the control of the isopropyl -D-thiogalactopyranoside (IPTG)-dependent Ptrc promoter.. A low level of IPTG induced a nontoxic but sufficient level of F to interact with the core enzyme of RNA polymerase.

Article Title: Mapping of transcription start sites of human retina expressed genes
Article Snippet: First strand cDNA synthesis was performed using Avian Myeloblastoma Virus (AMV) reverse transcriptase and 5× AMV-RT buffer (Promega) according to the manufacturer's instructions in a final volume of 60 μl.

Virus:

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA
Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Article Title: Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containingMycobacterium tuberculosis stress response σ factor, σF
Article Snippet: The previously established two-plasmid system in Escherichia coli for the identification of Mycobacterium tuberculosis promoters that are recognized by RNA polymerase containing the stress response factor F was optimized.. Expression of the M. tuberculosis F encoded by sigF gene was under the control of the isopropyl -D-thiogalactopyranoside (IPTG)-dependent Ptrc promoter.. A low level of IPTG induced a nontoxic but sufficient level of F to interact with the core enzyme of RNA polymerase.

Article Title: Mapping of transcription start sites of human retina expressed genes
Article Snippet: First strand cDNA synthesis was performed using Avian Myeloblastoma Virus (AMV) reverse transcriptase and 5× AMV-RT buffer (Promega) according to the manufacturer's instructions in a final volume of 60 μl.

Reverse Transcription:

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA
Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Article Title: Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containingMycobacterium tuberculosis stress response σ factor, σF
Article Snippet: The previously established two-plasmid system in Escherichia coli for the identification of Mycobacterium tuberculosis promoters that are recognized by RNA polymerase containing the stress response factor F was optimized.. Expression of the M. tuberculosis F encoded by sigF gene was under the control of the isopropyl -D-thiogalactopyranoside (IPTG)-dependent Ptrc promoter.. A low level of IPTG induced a nontoxic but sufficient level of F to interact with the core enzyme of RNA polymerase.

Article Title: Mapping of transcription start sites of human retina expressed genes
Article Snippet: First strand cDNA synthesis was performed using Avian Myeloblastoma Virus (AMV) reverse transcriptase and 5× AMV-RT buffer (Promega) according to the manufacturer's instructions in a final volume of 60 μl.



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Incorporation of T opposite 7-EAA and 7-EAA triazole by <t>AMV-RT</t> reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing <t>different</t> <t>ddNTPs</t> and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.
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Incorporation of T opposite 7-EAA and 7-EAA triazole by <t>AMV-RT</t> reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing <t>different</t> <t>ddNTPs</t> and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.
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Incorporation of T opposite 7-EAA and 7-EAA triazole by <t>AMV-RT</t> reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing <t>different</t> <t>ddNTPs</t> and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.
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Incorporation of T opposite 7-EAA and 7-EAA triazole by <t>AMV-RT</t> reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing <t>different</t> <t>ddNTPs</t> and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.
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Incorporation of T opposite 7-EAA and 7-EAA triazole by <t>AMV-RT</t> reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing <t>different</t> <t>ddNTPs</t> and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.
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Image Search Results


Incorporation of T opposite 7-EAA and 7-EAA triazole by AMV-RT reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing different ddNTPs and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.

Journal: ACS Chemical Biology

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA

doi: 10.1021/cb500270x

Figure Lengend Snippet: Incorporation of T opposite 7-EAA and 7-EAA triazole by AMV-RT reaction. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide (A, 7-EAA, or 7-EAA triazole). (B–D) Primer extension results for reactions containing different ddNTPs and different template strands, with N = A, 7-EAA, or 7-EAA-triazole.

Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Techniques: Primer Extension Assay

Controlling the extent of pausing by AMV-RT at a 7-EAA site. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide. (B) Primer extension results for reactions containing 10 μM dNTPs at 42 °C for 45 min. Lanes are labeled as follows for N: −, labeled primer only, no extension; A, adenosine; 7, 7-EAA; B, biotin triazole; and B + S, biotin triazole + monomeric streptavidin. (C) Primer extension results for reactions containing 1 μM dNTPs at 37 °C for 5 min; lanes are labeled the same as in panel B, and the arrow indicates a pause site. (D) Quantification of inhibition of primer extension under the conditions used in panel C; the average for at least three independent primer extension reactions ± standard deviation is plotted.

Journal: ACS Chemical Biology

Article Title: Click Modification of RNA at Adenosine: Structure and Reactivity of 7-Ethynyl- and 7-Triazolyl-8-aza-7-deazaadenosine in RNA

doi: 10.1021/cb500270x

Figure Lengend Snippet: Controlling the extent of pausing by AMV-RT at a 7-EAA site. (A) Sequences of strands employed in primer extension assay, with N indicating the site of the variable nucleotide. (B) Primer extension results for reactions containing 10 μM dNTPs at 42 °C for 45 min. Lanes are labeled as follows for N: −, labeled primer only, no extension; A, adenosine; 7, 7-EAA; B, biotin triazole; and B + S, biotin triazole + monomeric streptavidin. (C) Primer extension results for reactions containing 1 μM dNTPs at 37 °C for 5 min; lanes are labeled the same as in panel B, and the arrow indicates a pause site. (D) Quantification of inhibition of primer extension under the conditions used in panel C; the average for at least three independent primer extension reactions ± standard deviation is plotted.

Article Snippet: For each reaction, dNTPs, ddNTPs, 5× Promega AMV-RT buffer, and AMV-RT were added so that the concentrations in the reaction were as follows: 20 nM RNA, ∼80 nM 32 P-labeled 21 nt GluR B pre-mRNA primer, 10 mM of the 3 dNTPs, 10 mM of ddNTP for the fourth base, 1× Promega AMV-RT buffer, and 5 units of AMV-RT.

Techniques: Primer Extension Assay, Labeling, Inhibition, Standard Deviation